Lupine rapid test SENSIStrip Powerline

NEW

For surfaces, rinse water and product analysis, swabs included
Improved and faster procedure compared to the 2-line SENSIStrip test kits

Price per piece, available per 5 pieces or multiples of 5.

Content

• test strip
• Swab
• Extraction tube (transparent cap)
• Reaction tube (blue cap)
• Bottle with extraction buffer
• Cover it up
• Large pipette 
• Pipette small (0,3 ml)
• Cardboard holders
• QR code for use with the Reader (Optional)

Detection limit

Product: 0,18 ppm lupine protein, 0,5 ppm lupine
Swab/environmental study: 0,04 µg/swab lupine protein, 0,1 µg/swab lupine
Rinsing water: 0,05 mg/l lupine protein, 0,07 mg/l lupine 
Protein conversion factor: 0,36 (lupine contains 36% protein)
Sensitivity depends on the type of food, the amount of product under investigation and the extraction method. Contact us for a determination of the detection limit in a specific product.

Operating principle

Using specific antibodies (similar to ELISA tests), the presence of the allergen being tested is made visible by the appearance of a line. The test strip contains three lines: a control line that indicates whether the test has been performed correctly, a test line, and a hook line. 
The principle is comparable to a corona or pregnancy test.

To use for

> Securing the chain and checking suppliers 
> Control raw materials
> Determine and verify cross-contamination 
> Control cleaning 
> Handling rework 
> Test end products
> Check rinse water

Additional information

The detection limit varies per specific lupine species, Lupinus anugustifolius and Lupinus alba have a slightly increased LOD compared to Luteus luteus. 

During product analysis, it is important that the test strip does not become blocked by product residues or thickeners. If the liquid cannot flow properly through the strip, it will not reach the C-line, resulting in an invalid result. Therefore, allow solid product residues to settle thoroughly before testing. For products containing thickeners or that are highly viscous, it may be necessary to dilute the sample with water beforehand. Please note that dilution affects the detection limit.
In the analysis of products with a high content of polyphenols, such as pure spices, cocoa (powder), nuts, seeds, or berries, the use of a special extraction additive necessary. The additive prevents false-positive test results by binding polyphenols.

For both product and rinse water analyses, the pH of the sample must be between 6 and 8. Abnormal results may occur outside this pH range. At a low pH, there is a risk of a false-positive result.
A buffer solution is supplied with the test to stabilize the pH and salt concentration. The amount of buffer is matched to the prescribed sample quantity. It is therefore essential to accurately weigh the sample during product analysis, for example with the precision balance.

Contact us for more information about customized extraction protocols, for example for strongly colored foods.
It is recommended to validate the method by using both a negative and positive monster to analyze the product to be investigated. Dilute the positive sample to the desired measurement range. 

Simple working method

Download
Manual

Store 2 – 8°C 
Shelf life approx. 12 months (min. 3 months upon delivery)

Quantify results with reader (optional)

The strips can be easily visually assessed. No additional equipment is required. This determines whether the allergen is detectable or not.
Optionally, the strips can also be read with the separately available readerThis reader measures the intensity of the lines on the strip and calculates the allergen content in ppm (parts per million or mg per kg) based on that information. This method is similar to an ELISA analysis, but faster and more user-friendly. Accredited external analyses may still be necessary, but the reader saves time and money by performing the measurements yourself.
The reading with the reader gives the following values:

If the concentration is higher than 25 ppm, a 1:10 dilution can be made. This extends the measuring range by a factor of 10 to 5-250 ppm (or even higher with additional dilutions). Dilutions are made with the extraction buffer included in the kit. The dilution procedure can be found in the included manual.

Important: ppm values ​​(mg/kg product) are only relevant when using the strips for product testing. These values ​​can be compared with product standards, such as PAL (Precautionary Allergen Labeling). action limits for warnings on labels.

overview
measuring ranges 

We do not recommend using the reader for swabs (surface tests) and rinse water. Swabs sample cm², and rinse water is not consumed. Therefore, the amount of allergen on a surface or in rinse water cannot be expressed in mg/kg product. Therefore, the read values ​​cannot be compared with product standards.  

More information about the SENSIStrip rapid test

SENSIStrip is a trademark of Gold Standard Diagnostics Budapest Kft.